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CHAPTER 1 - THE CELL
Histology Guide
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MICROGRAPH

NAME
EM 008 Golgi Apparatus
TISSUE
β-cell
Islet of Langerhans
(rat pancreas)
IMAGE SIZE
5,240 x 4,503 pixels
90 MB
FILE SIZES
23,500 KB (grayscale)
23,596 KB (color)
MAGNIFICATION
74,286x
PIXEL SIZE
0.6011 nm
SOURCE
Stanley L. Erlandsen
Department of Genetics, Cell Biology and Development
School of Medicine
University of Minnesota
Minneapolis, MN

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EM 008 Golgi Apparatus

Golgi Apparatus

This is the Golgi apparatus from a pancreatic β cell as seen by transmission electron microscopy (TEM).

Pro-Insulin is synthesized on rough endoplasmic reticulum, transported to the Golgi apparatus by transfer vesicles, moves through the stack of flattened cisterna, and packaged into secretion granules.

Examine the morphology of the following structures:

  • (yellow) - stack of flattened cisterna.
  • (purple) - transfer newly synthesized proteins from the endoplasmic reticulum to the Golgi apparatus.
  • (gray) - the content of immature granules is homogenous, while mature granules have an electron-dense crystal of insulin (black). The mature granules are 250 to 300 nm in diameter.

(The morphology of secretion granules varies widely in different cell types.)

Other structures:

  • Nucleus (blue)
    • Nuclear Envelope (purple)
  • Mitochondria (red)
  • Endoplasmic Reticulum (cyan)
  • Cytoplasm (green)
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